Specific NEMO mutations impair CD40-mediated c-Rel activation and B cell terminal differentiation
Identifieur interne : 008712 ( Main/Exploration ); précédent : 008711; suivant : 008713Specific NEMO mutations impair CD40-mediated c-Rel activation and B cell terminal differentiation
Auteurs : Ashish Jain [États-Unis] ; Chi A. Ma [États-Unis] ; Eduardo Lopez-Granados [États-Unis] ; Gary Means [États-Unis] ; William Brady [États-Unis] ; Jordan S. Orange [États-Unis] ; Shuying Liu [États-Unis] ; Steven Holland [États-Unis] ; Jonathan M. J. Derry [États-Unis]Source :
- Journal of Clinical Investigation [ 0021-9738 ] ; 2004.
Abstract
Hypomorphic mutations in the zinc finger domain of NF-κB essential modulator (NEMO) cause X-linked hyper-IgM syndrome with ectodermal dysplasia (XHM-ED). Here we report that patient B cells are characterized by an absence of Ig somatic hypermutation (SHM) and defective class switch recombination (CSR) despite normal induction of activation-induced cytidine deaminase (AID) and Iε-Cε transcripts. This indicates that AID expression alone is insufficient to support neutralizing antibody responses. Furthermore, we show that patient B cells stimulated with CD40 ligand are impaired in both p65 and c-Rel activation, and whereas addition of IL-4 can enhance p65 activity, c-Rel activity remains deficient. This suggests that these NF-κB components have different activation requirements and that IL-4 can augment some but not all NEMO-dependent NF-κB signaling. Finally, using microarray analysis of patient B cells we identified downstream effects of impaired NF-κB activation and candidate factors that may be necessary for CSR and SHM in B cells.
Url:
DOI: 10.1172/JCI200421345
PubMed: 15578091
PubMed Central: 529497
Affiliations:
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<front><div type="abstract" xml:lang="en"><p>Hypomorphic mutations in the zinc finger domain of NF-κB essential modulator (NEMO) cause X-linked hyper-IgM syndrome with ectodermal dysplasia (XHM-ED). Here we report that patient B cells are characterized by an absence of Ig somatic hypermutation (SHM) and defective class switch recombination (CSR) despite normal induction of activation-induced cytidine deaminase (AID) and Iε-Cε transcripts. This indicates that AID expression alone is insufficient to support neutralizing antibody responses. Furthermore, we show that patient B cells stimulated with CD40 ligand are impaired in both p65 and c-Rel activation, and whereas addition of IL-4 can enhance p65 activity, c-Rel activity remains deficient. This suggests that these NF-κB components have different activation requirements and that IL-4 can augment some but not all NEMO-dependent NF-κB signaling. Finally, using microarray analysis of patient B cells we identified downstream effects of impaired NF-κB activation and candidate factors that may be necessary for CSR and SHM in B cells.</p>
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